Difference between revisions of "Part:BBa K2984030"

 
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<partinfo>BBa_K2984030 short</partinfo>
 
<partinfo>BBa_K2984030 short</partinfo>
  
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L1 construc containg Arylsulfatase secretion Signal, mVenus fluorescent marker, SP20 glycomodule. These parts are flanked by the Psad Promoter and the Rbcs2 Terminator. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1 construc containg Arylsulfatase secretion Signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> fluorescent marker, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule. These parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:18, 17 October 2019


L1c-Psad-ARS-YFP-SP20-Rbcs2

This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1 construc containg Arylsulfatase secretion Signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> fluorescent marker, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule. These parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]