Difference between revisions of "Part:BBa K2984027"

 
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<partinfo>BBa_K2984027 short</partinfo>
 
<partinfo>BBa_K2984027 short</partinfo>
  
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L1c plasmid containg arylsulfatase secretion signal and mVEnus fluorescent marker which are flanked by Psad Promoter and Rbcs2 Terminator. The construct is designed to test for the efficency of the secretion signal voa fluorescence intensity measurements.
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L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.
  
  
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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==Characterization==
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<html>
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<figure>
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<img src="igem" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
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</figure>
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</html>
  
 
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Revision as of 08:40, 17 October 2019


L1c-Psad-ARS-YFP-Rbcs2

L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]