Difference between revisions of "Part:BBa K3089021"
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This composite parts is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5 is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesive properties by displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.  This part was designed based on the core part——mfp5, which has been submitted into parts registry by iGEM2015 Tu_delft(BBa_K1583002).
 
This composite parts is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5 is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesive properties by displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.  This part was designed based on the core part——mfp5, which has been submitted into parts registry by iGEM2015 Tu_delft(BBa_K1583002).
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This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:45, 16 October 2019


T7 promoter+csgA-linker-mfp5-His fusion protein

This composite parts is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5 is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesive properties by displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications. This part was designed based on the core part——mfp5, which has been submitted into parts registry by iGEM2015 Tu_delft(BBa_K1583002).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 314
  • 1000
    COMPATIBLE WITH RFC[1000]