Difference between revisions of "Part:BBa K3038007:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
RFC10 design  
 
RFC10 design  
 +
 
Optimized for GC content  
 
Optimized for GC content  
 +
 
6His tag added at the Cterm part (bp 3159 to 3176)
 
6His tag added at the Cterm part (bp 3159 to 3176)
  

Latest revision as of 09:40, 16 October 2019


Cytochrome 102A1 BM3 Mutant S72Y V78A F87A _Cterm 6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1225
    Illegal SapI.rc site found at 2248
    Illegal SapI.rc site found at 2848


Design Notes

RFC10 design

Optimized for GC content

6His tag added at the Cterm part (bp 3159 to 3176)

Source

sequence synthesis by IDT

References