Difference between revisions of "Part:BBa K3089021"

 
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<partinfo>BBa_K3089021 short</partinfo>
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This composite part is meant to express csgA-linker-mfp5  fusion genes under T7 promoter and purify CsgA-linker-Mfp5  recombinant proteins by His-tag affinity purification.  CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion.  This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.
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This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3089021 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3089021 parameters</partinfo>
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Revision as of 09:39, 16 October 2019


T7 promoter+csgA-linker-mfp5-His fusion protein

This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.

This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 314
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 314
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 314
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 314
  • 1000
    COMPATIBLE WITH RFC[1000]