Difference between revisions of "Part:BBa K3190601"
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The coding sequence of BAX Inhibitor-I anti-toxin under the control of inducible promoter for the design of a kill switch system for a yeast wherein a BAX toxin is expressed under a constitutive promoter. This system ensures that the yeast survives only unless it is present in the medium with inducer. | The coding sequence of BAX Inhibitor-I anti-toxin under the control of inducible promoter for the design of a kill switch system for a yeast wherein a BAX toxin is expressed under a constitutive promoter. This system ensures that the yeast survives only unless it is present in the medium with inducer. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[File:UCopenhagen Killswitch.jpeg|600px]] | ||
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| + | <small> Figure legend: Overview of the toxin/anti-toxin biosafety device. </small> | ||
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Revision as of 11:53, 16 October 2019
pGAL1-BI-I: BI-I CDS under inducible promoter
The coding sequence of BAX Inhibitor-I anti-toxin under the control of inducible promoter for the design of a kill switch system for a yeast wherein a BAX toxin is expressed under a constitutive promoter. This system ensures that the yeast survives only unless it is present in the medium with inducer.
Usage and Biology
Figure legend: Overview of the toxin/anti-toxin biosafety device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 954
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 377
- 1000COMPATIBLE WITH RFC[1000]