Difference between revisions of "Part:BBa K3110048:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli | + | Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. |
===Source=== | ===Source=== | ||
Revision as of 10:23, 16 October 2019
IL12 FLAG-tag (Codon Optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli.
Source
Sequence was synthesized by Twist Bioscience.