Difference between revisions of "Part:BBa K2927015"
 
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This part encode the crRNA-5 that mediates Cas12a protein to recognize target sequences. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity). The cleavage of double-stranded DNA activates the ability of Cas12a protein to cleave non-specific ssDNA (trans cleavage activity). In our project, crRNA-5 is designed to detect specific sequence of vp72 gene in African swine fever virus (ASFV).
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This part encode the crRNA-6 that mediates Cas12a protein to recognize target sequences. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity). The cleavage of double-stranded DNA activates the ability of Cas12a protein to cleave non-specific ssDNA (trans cleavage activity). In our project, crRNA-6 is designed to detect specific sequence of vp72 gene in African swine fever virus (ASFV).
  
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Of note, crRNA-5 only contains 20 nucleotide sequences from vp72 gene of ASFV, which shows no potential of protein coding. Our design had been reported to iGEM and got approved.  
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Of note, crRNA-6 only contains 20 nucleotide sequences from vp72 gene of ASFV, which shows no potential of protein coding. Our design had been reported to iGEM and got approved.  
 
 
 
 
  

Latest revision as of 05:49, 16 October 2019


DNA template of crRNA 6 for LbCas12a


This part encode the crRNA-6 that mediates Cas12a protein to recognize target sequences. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity). The cleavage of double-stranded DNA activates the ability of Cas12a protein to cleave non-specific ssDNA (trans cleavage activity). In our project, crRNA-6 is designed to detect specific sequence of vp72 gene in African swine fever virus (ASFV).

Of note, crRNA-6 only contains 20 nucleotide sequences from vp72 gene of ASFV, which shows no potential of protein coding. Our design had been reported to iGEM and got approved.    


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]