Difference between revisions of "Part:BBa K2950002"

 
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Toluene dioxygenase is an ISP protein identified from Pseudomonas putida which contains alpha subunit(TodC1) and beta subunit(TodC2). TodC1 carries the oxidation reaction of toluene under the presence of Tod C2.  
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<p>In our design, TodC1C2 is utilized to oxidize hexadecylbenzene to the intermediate cis-hexadecylbenzene dihydrodiol. As a protein with quaternary structure, TodC1C2 contains two polypeptides, todC1 and TodC2, with the size of 53 and 25 kDa. We demonstrated this part via SDS-PAGE electrophoresis, as shown in the figure below.</p>
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<img src="https://2019.igem.org/wiki/images/c/cd/T--BNDS_China--todC12.jpeg">
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:19, 21 October 2019


Toluene dioxygenase(TodC1C2)

In our design, TodC1C2 is utilized to oxidize hexadecylbenzene to the intermediate cis-hexadecylbenzene dihydrodiol. As a protein with quaternary structure, TodC1C2 contains two polypeptides, todC1 and TodC2, with the size of 53 and 25 kDa. We demonstrated this part via SDS-PAGE electrophoresis, as shown in the figure below.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
    Illegal BglII site found at 714
    Illegal BglII site found at 729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1527
    Illegal NgoMIV site found at 82
    Illegal NgoMIV site found at 1384
    Illegal NgoMIV site found at 1905
    Illegal NgoMIV site found at 1947
    Illegal AgeI site found at 1511
  • 1000
    COMPATIBLE WITH RFC[1000]