Difference between revisions of "Part:BBa K3239007"
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This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination. | This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination. | ||
− | [[File:pAOX1-GFP.png|200px|thumb|Figure 1. Illustration of the | + | [[File:pAOX1-GFP.png|200px|thumb|Figure 1. Illustration of the pAOX1-GFP construct]] |
Revision as of 05:34, 16 October 2019
pAOX1-GFP
yEFGP3 reporter gene expressed by pAOX1.
Usage and Biology
This construct is the control used in our experiment. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 935
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]