Difference between revisions of "Part:BBa K2926057"

 
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Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.
 
Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h1>Sequence and Features<h1></span>
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Sequence was validated by Sanger sequencing.
 
<partinfo>BBa_K2926057 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2926057 SequenceAndFeatures</partinfo>
  

Revision as of 22:54, 17 October 2019


Fusion protein of mating factor alpha, mCherry and pVIII with his-tag

Mating factor alpha from S. cerevisiae was fused n-terminally to the red fluorescent protein mCherry. Additionally the M13 bacteriophages major coat protein pVIII was fused to the c-terminus of mCherry. To enable easy purificatio of the fusion protein a hexahistidine tag was fused to the c-terminus.


Sequence and Features<h1></span> Sequence was validated by Sanger sequencing.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]