Difference between revisions of "Part:BBa K3187028"
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<partinfo>BBa_K3187028 short</partinfo> | <partinfo>BBa_K3187028 short</partinfo> | ||
| − | <p>Sortase A7M (SrtA7M) is a calcium-independent transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA7M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA7M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a 6xHis-Tag downstream of the coding sequence were added. | + | <p>Sortase A7M (SrtA7M) is a calcium-independent transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA7M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA7M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a 6xHis-Tag downstream of the coding sequence were added. </p> |
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Revision as of 12:26, 16 October 2019
Sortase A7M (Ca2+-independent variant)
Sortase A7M (SrtA7M) is a calcium-independent transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA7M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA7M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a 6xHis-Tag downstream of the coding sequence were added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]