Difference between revisions of "Part:BBa K3102032:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We mutated a Thymine into Cysteine at the | + | We mutated the ZWF sequence with a Thymine into Cysteine at the 799bp position (T799C) because of the PstI enzyme (BBa_K3102008) |
===Source=== | ===Source=== |
Latest revision as of 10:48, 16 October 2019
T7-RBS-ZWF-Ter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 810
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 810
Illegal NotI site found at 691 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1367
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 810
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 810
Illegal NgoMIV site found at 83
Illegal NgoMIV site found at 800 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated the ZWF sequence with a Thymine into Cysteine at the 799bp position (T799C) because of the PstI enzyme (BBa_K3102008)
Source
Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Zhao J. et al., "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Metab. Eng. 6:164-174(2004)