Difference between revisions of "Part:BBa K2996709"

Revision as of 16:23, 19 October 2019

LuxI downstream of activation unit containing lure

LuxI is put downstream of BBa_K2996704, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL). We develop this to record off-target incidence. When the dcas9-sgRNA complex binds to target sequence, RpoA will recruit RNA polymerase, promoter J23117 will be activated. Then, by measuring the biological activity of the downstream reporter gene, we will know the effectiveness of this transcription activation device under different conditions.

Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 52
Illegal NheI site found at 75
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 743
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 39
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2996709 short</partinfo>
-LuxI is put downstream of BBa_K2996705, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL).
+LuxI is put downstream of BBa_K2996704, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL).
-dcas9 binding to lure means the occurrence of off-target incidence. RpoA, which is linked to dcas9, will recruits RNA polymerase and the gene downstream of the promotor will be activated.
+We develop this to record off-target incidence. When the dcas9-sgRNA complex binds to target sequence, RpoA will recruit RNA polymerase, promoter J23117 will be activated. Then, by measuring the biological activity of the downstream reporter gene, we will know the effectiveness of this transcription activation device under different conditions.
-We develop a way to record off-target incidence, with LuxI gene under the control of promotor J23117. Increased expression of luxI enzyme will synthesis enough AHL molecules, which can penetrate to the bacteria capable of information storage.
+Increased expression of luxI enzyme indicates the off-target incidence, and AHL molecules, synthesized by LuxI, will penetrate to the bacteria capable of information storage.