Difference between revisions of "Part:BBa K3040107"
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| − | + | Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid. | |
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Revision as of 16:08, 16 October 2019
TesA
Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 981
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 982
Illegal PstI site found at 996
Illegal NotI site found at 989 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 982
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 982
Illegal PstI site found at 996 - 1000COMPATIBLE WITH RFC[1000]