Difference between revisions of "Part:BBa K2912015"
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===Usage and Biology===
 
===Usage and Biology===
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The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the  interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene
  
  

Revision as of 17:23, 15 October 2019


Trp_Lysis gene

Trp_Lysis gene expression can be controlled by the concentration of Tryptophan

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene