Difference between revisions of "Part:BBa K2984024"
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| − | For <i>in vivo</i> detection of proteins in <i>C. reinhardtii</i> or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for <i>C. reinhardtii</i> as it can be detected with a fluorescence microscope. | + | <html> |
| + | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. For <i>in vivo</i> detection of proteins in <i>C. reinhardtii</i> or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for <i>C. reinhardtii</i> as it can be detected with a fluorescence microscope. | ||
| + | </html> | ||
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Revision as of 14:11, 17 October 2019
YFP - Yellow fluorescent protein for detection B4-B4
This part is a part of the Chlamy-HUB-Collection. For in vivo detection of proteins in C. reinhardtii or its surroundings. The green fluorescent protein variant carries amino acid substitutions that shift the protein to which it is bound to an emission peak at 530 nm seen as yellow (Ormo et al., 1996). For detection of fluorescence it is not necessary to process a sample nor adding a substance to make it visible making it safe for C. reinhardtii as it can be detected with a fluorescence microscope.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 220
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 220
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 220
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 220
Illegal NgoMIV site found at 697 - 1000COMPATIBLE WITH RFC[1000]