Difference between revisions of "Part:BBa K3279008:Design"
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===Source===
 
===Source===
  
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cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae's genomic sequence and we did a codon optimization.  
+
cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae genomic DNA and we (CAU_China 2019) did a codon optimization.
  
 
===References===
 
===References===

Revision as of 15:22, 15 October 2019


cenA gene fused with INP-N sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
    Illegal AgeI site found at 1051
    Illegal AgeI site found at 1414
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primary concern was BioBrick compatibility so that illegal sites in RFC[10] did not appear in the sequence. And the sequeces was finally designed to link with pET30a(+) , so we added restriction enzyme cutting site NdeI at the 5' and SalI at the 3'.


Source

cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae genomic DNA and we (CAU_China 2019) did a codon optimization.

References