Difference between revisions of "Part:BBa K3037000"
Line 35: Line 35:
  
  
−
This BioBrick was site mutated to take out a XbaI restriction site
+
This BioBrick was designed to fit the RFC 10 standard using the primers:
 +
 
 +
Forward: tactagtagcggccgctgcagCCGTTATAGAAGCTTGAGTATT
 +
Reverse: gaattcgcggccgcttctagagGCCCATGGATATATCTCCTTCT
 +
 
 +
Also the expression was optimized as shown in the characterization in the main page

Revision as of 22:07, 16 October 2019

pOCC97 plasmid backbone for expression (optimized)

pOCC97
Function Expression
Use in Escherichia coli T7
RFC standard RFC 10 compatible
Submitted by Team:TU_Dresden 2019[1]

Overview

The TU Dresden 2019 team design this biobrick in order to express its fusion protein (more information)


Biology

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5283
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5283
    Illegal BglII site found at 5169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5283
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5283
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5298
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 342
    Illegal NgoMIV site found at 3389
    Illegal NgoMIV site found at 3549
    Illegal NgoMIV site found at 5137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2468


Design Notes

This BioBrick was designed to fit the RFC 10 standard using the primers:

Forward: tactagtagcggccgctgcagCCGTTATAGAAGCTTGAGTATT Reverse: gaattcgcggccgcttctagagGCCCATGGATATATCTCCTTCT

Also the expression was optimized as shown in the characterization in the main page