Difference between revisions of "Part:BBa K3195004"
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===Usage and Biology===
 
===Usage and Biology===
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Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid.
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Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid. In industry subtilisin has been widely used in all walks of life. Interestingly, we found the subtilisin-like protease can degrade not only proteins but also microcystins. Then we test the activity of this enzyme and measured by HPLC-MS-MS
  
 
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Revision as of 15:49, 15 October 2019


Trx-His-EK tagged subtilisin-like protease

Subtilisin-like protease is a kind of alcalase found in amphioxus (Branchiostoma) similar to subtilisin protease. In our project, it has been used to degrade microcystin with another enzyme called carboxypeptidase Z/N.

Usage and Biology

Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid. In industry subtilisin has been widely used in all walks of life. Interestingly, we found the subtilisin-like protease can degrade not only proteins but also microcystins. Then we test the activity of this enzyme and measured by HPLC-MS-MS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
    Illegal XhoI site found at 1645
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 967