Difference between revisions of "Part:BBa K2926067"
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<partinfo>BBa_K2926067 short</partinfo> | <partinfo>BBa_K2926067 short</partinfo> | ||
| − | The extracellular cysteine-rich domain of the membrane protein Opy2 from S. cerevisiae was n-terminally fused to the red fluorescing reporter protein mCherry. Additionally, the M13 bacteriophage major coat protein pVIII was fused to the | + | The extracellular cysteine-rich domain of the membrane protein Opy2 from <i>S. cerevisiae</i> was n-terminally fused to the red fluorescing reporter protein mCherry. Additionally, the M13 bacteriophage major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy protein purification a hexahistidine tag was fused to the C-terminus of the protein. |
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><h1>Sequence and Features</h1></span> |
| + | Sequence was validated by Sanger sequencing. | ||
<partinfo>BBa_K2926067 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2926067 SequenceAndFeatures</partinfo> | ||
Latest revision as of 22:44, 17 October 2019
Fusion protein of Opy2 (yeast), mCherry and pVIII (bacteriophage M13) with purification tag
The extracellular cysteine-rich domain of the membrane protein Opy2 from S. cerevisiae was n-terminally fused to the red fluorescing reporter protein mCherry. Additionally, the M13 bacteriophage major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy protein purification a hexahistidine tag was fused to the C-terminus of the protein.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]