Difference between revisions of "Part:BBa K2624000"

Revision as of 12:16, 15 October 2019

hTERT promoter

It's the core promoter of human telomerase reverse transcriptase (hTERT) gene. And it's cancer-specific. If you want to do something specifically to cancer cells, for them to express certain product, you may need it.

Usage

The 2018 CPU_CHINA project is an AND-gated system targeting hepatocellular carcinoma. HTERT is one of the most known cancer-specific promoters, suitable for many cancer cell-related scheme. With this promoter, it’s more likely that normal cells would not express the protein contents that come after it. Only tumor cells (malignant ones especially) express NS5B and ‘open’ the AND gate, releasing miRNA that damage themselves. aaaa

Biology

Telomerase activation maintains telomeres and is critical for human carcinogenesis. Telomerase is a reverse transcriptase enzyme that carries its own RNA molecule. Transcriptional regulation of the enzyme part (hTERT) gene has been found to be the major mechanism for cancer-specific activation of telomerase. A number of factors have been identified to directly or indirectly regulate the hTERT promoter, which include famous cellular transcriptional activators such as c-Myc, Sp1, HIF-1, AP2, ER, as well as the repressors p53, WT1, and Menin, most of which comprise tumor suppressor gene products.
The chromatin structure via the DNA methylation or modulation of nucleosome histones is also important for regulation of the hTERT promoter. DNA unmethylation or histone methylation around the transcription start site of the hTERT promoter triggers the recruitment of histone acetyltransferase (HAT) activity, allowing hTERT transcription.

Results

Sequence and Features