Difference between revisions of "Part:BBa K1033910"

Revision as of 02:10, 22 October 2019

fwYellow, yellow chromoprotein

This chromoprotein (also known as FezziwigYFP) naturally exhibits strong color when expressed. Compared to many other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-64 hours of incubation.

Usage and Biology

This part is useful as a reporter.

iGEM13_Uppsala: Expression of chromoproteins. The images above show E coli constitutively expressing meffRed BBa_K1033922, aeBlue BBa_K1033929, tsPurple BBa_K1033905, asPink (asCP) BBa_K1033927, fwYellow BBa_K1033910, meffBlue BBa_K1033902, meffRed BBa_K1033922 and scOrange BBa_K1033913.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 109
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 486

Characterization: NWU-China 2019

The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).

Figure 1. fwYellow absorbance spectrum

At the same time, fwYellow can emit strong fluorescence under blue light excitation.

Figure 1. under blue light excitation, The left one is fwYellow

When we used the microplate reader to measure the excitation light and absorbed light, we found that the spectrum of fwYellow is too narrow. Due to the low precision of the instrument, it is impossible to accurately measure its excitation light and absorption light. The excitation light is around 522 nm and the emission is about 536 nm.

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 <br>
 At the same time, fwYellow can emit strong fluorescence under blue light excitation.
-[[File:T--NWU-China--Characterization-yellow.jpg|900px|thumb|none|alt=fluorescence of fwYellow.|Figure 1. under blue light excitation, The left one is fwYellow]]
+[[File:T--NWU-China--Characterization-yellow.jpg|900px|thumb|none|alt=fluorescence of fwYellow.|Figure 1. under blue light excitation, The left one is fwYellow]]<br>
+<br>
+When we used the microplate reader to measure the excitation light and absorbed light, we found that the spectrum of fwYellow is too narrow. Due to the low precision of the instrument, it is impossible to accurately measure its excitation light and absorption light. The excitation light is around 522 nm and the emission is about 536 nm.
 ;