Difference between revisions of "Part:BBa K3096052"
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<partinfo>BBa_K3096052 short</partinfo> | <partinfo>BBa_K3096052 short</partinfo> | ||
This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded. | This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded. | ||
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+ | ===Sequence and Features=== | ||
+ | <partinfo>BBa_K3096052 SequenceAndFeatures</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 14:50, 15 October 2019
CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system
This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]