Difference between revisions of "Part:BBa K2984020:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Codonoptimization of part, Use of promoter, terminator respectively that originates from targeted locus.
 
  
 +
This was designed as a B5-B5 part according to the MoClo standard of Patron et al. (2015).
  
  
 
===Source===
 
===Source===
  
−
Promoter ist part of 5'UTR of photosystemI reaction center subunit II-1. The terminator originates form 3'UTR Ribulose-1,5-bisphosphat-carboxylase.
+
Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395.
  
 
===References===
 
===References===
 +
 +
Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395.
 +
Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.

Latest revision as of 16:28, 15 October 2019


YFP - Yellow fluorescent protein for detection B5-B5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This was designed as a B5-B5 part according to the MoClo standard of Patron et al. (2015).


Source

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395.

References

Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., & Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science, 273(5280), 1392-1395. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.