Difference between revisions of "Part:BBa K2947000"
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The protoplast and salidroside standard samples of arabidopsis thaliana transfected with UGT33 and 4HPAAS genes were compared by liquid chromatography. The peak value of the product measured in sample No. 1 was basically consistent with the peak value of salidroside standard samples. | The protoplast and salidroside standard samples of arabidopsis thaliana transfected with UGT33 and 4HPAAS genes were compared by liquid chromatography. The peak value of the product measured in sample No. 1 was basically consistent with the peak value of salidroside standard samples. | ||
| − | <html><img src="https://2019.igem.org/wiki/images/1/17/T--GDSYZX--demonstrate-1.png" width=" | + | <html><img src="https://2019.igem.org/wiki/images/1/17/T--GDSYZX--demonstrate-1.png" width="800" height="300" BORDER=0</ a></html></br> |
Therefore, we can determine that the transfected protoplast of arabidopsis thaliana can produce salidroside. | Therefore, we can determine that the transfected protoplast of arabidopsis thaliana can produce salidroside. | ||
Revision as of 10:31, 15 October 2019
4HPAAS
4HPAAS(referring to 4-hydroxyphenylacetaldehyde synthase) protein coding region. It reduces L-tyrosine to produce 4-hydroxy-phenylacetaldehyde. It is the first enzyme in the biosynthesis of salidroside.
Usage and Biology
4HPAAS is one gene of the Aromatic acetaldehyde synthases(AASs). AASs, together with tryptophan decarboxylases(TDCs) and TyDCs, encompass a large family of pyridoxal phosphate(PLP)-dependent enzymes broadly referred to as the plant AAAD family.(Facchini et al., 2000; Kaminaga et al., 2006) As their respective names imply, TyDCs, YDCs, and AASs catalyze discrete decarboxylation or decarboxylation-deamination reactions using specific aromatic amino acids as substrates.
Previous research has shown that 4HPAAS functions in the direct conversion of tyrosin to 4-hydroxyphenylacetaldehyde(4-HPAA) in Rhodiola salidroside biosynthesis.(Michel et al., 2017)
The protoplast and salidroside standard samples of arabidopsis thaliana transfected with UGT33 and 4HPAAS genes were compared by liquid chromatography. The peak value of the product measured in sample No. 1 was basically consistent with the peak value of salidroside standard samples.
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Therefore, we can determine that the transfected protoplast of arabidopsis thaliana can produce salidroside.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 631
Illegal BglII site found at 712
Illegal BglII site found at 1156 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]