Difference between revisions of "Part:BBa K3286106"
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[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016. | [1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016. | ||
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| + | Chitin binding assay | ||
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| + | <html> | ||
| + | <figure> | ||
| + | <img style="width:50%;" src="https://2019.igem.org/wiki/images/2/23/T--Wageningen_UR--Partspage_BBa_K3286106.jpeg"> | ||
| + | <figcaption> | ||
| + | <b> Figure 1:</b> Protein concentration in supernatant in ug/ml. 55% of PD1764sh has bound to chitin in this assay. The influence of chitin on protein concentration in general is negligible, as the BSA sample indicates | ||
| + | </figcaption> | ||
| + | </figure> | ||
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Revision as of 14:25, 21 October 2019
Shorter version of PD1764
This protein is a shorter (sh) version of BBa_K3286105. Due to isolation troubles, the first 20 amino acids have been removed from this protein. The part contains a chitin-binding domain that is essential for chitin binding capacities [1].
[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.
Chitin binding assay
