Difference between revisions of "Part:BBa K3239003"
BrianSebZhou (Talk | contribs) (→Reference) |
BrianSebZhou (Talk | contribs) m (→Usage and Biology) |
||
Line 5: | Line 5: | ||
Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain. Derepressed by Mxr1 and upregulated by Mit1 and Prm1. | Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain. Derepressed by Mxr1 and upregulated by Mit1 and Prm1. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | The AOX1 promoter is | + | The AOX1 promoter is homogeneous to the P. pastoris GS115 strain. It is derepressed by Mxr1 and upregulated by Mit1 and Prm1, all transcription factors homogeneous to the P. pastoris GS115 strain. |
===Reference=== | ===Reference=== |
Revision as of 10:22, 15 October 2019
Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain
Promoter for the AOX1 (alcohol oxidase 1) gene in the P.pastoris GS115 strain. Derepressed by Mxr1 and upregulated by Mit1 and Prm1.
Usage and Biology
The AOX1 promoter is homogeneous to the P. pastoris GS115 strain. It is derepressed by Mxr1 and upregulated by Mit1 and Prm1, all transcription factors homogeneous to the P. pastoris GS115 strain.
Reference
Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738
Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014
Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]