Difference between revisions of "Part:BBa K2890001"

(We added the reporter gene GFP to the downstream of the promoter, then detected the sensitivity and tolerance of the promoter to formaldehyde using BL21 as chassis.)
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Contributions from Yuhan Sun, JNFLS 2019.
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Contributions from Yuhan Sun, iGEM2019_JNFLS
  
  

Revision as of 10:15, 15 October 2019


optimized frmR

A optimized frmR promoter for formaldehyde detection.


File:K2890001 contributions.docx


Contributions from Yuhan Sun, iGEM2019_JNFLS


We added the reporter gene GFP to the downstream of the promoter, then detected the sensitivity and tolerance of the promoter to formaldehyde using BL21 as chassis. We cultured E.coli BL21 transformed with the plasmid （PfrmR-GFP）and added different concentration formaldehyde to different groups when the OD600 = 1, then we collected bacteria at certain time to measure the fluorescence and the absorbance. From the Fig. 1, we found that 0.8 mM formaldehyde is the best concentration for expression reporter protein GFP.

K2890001 contribution.jpg

Fig.1 The sensitivity and tolerance of the promoter to formaldehyde.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 551