Difference between revisions of "Part:BBa K3239007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This is one of our constructs aimed at moderately upregulating pAOX1 activity in P.pastoris GS115. After homologous recombination into wild type P. pastoris, the EGFP expressed shall serve as an indicator of AOX1 expression levels. Note that the original pAOX1-AOX1 copy remains intact after the recombination. | ||
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| + | Both the promoter and the reporter are registered. | ||
| + | pAOX1: BBa_K3239003 | ||
| + | yEGFP3: BBa_K3239006 | ||
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<partinfo>BBa_K3239007 parameters</partinfo> | <partinfo>BBa_K3239007 parameters</partinfo> | ||
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Revision as of 10:31, 15 October 2019
pAOX1-GFP
yEFGP3 reporter gene expressed by pAOX1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 935
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]