Difference between revisions of "Part:BBa K3176020"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3176020 short</partinfo> | <partinfo>BBa_K3176020 short</partinfo> | ||
| − | Aptamers are short single-stranded DNA or RNA oligonucleotides that can be selected to a wide variety of targets. They are similar to antibodies through their affinity and specificity and show a high potential as diagnostic and therapeutic tools, with many advantages when compared with antibodies. BBa_K3176020 is a DNA aptamer selected against E. faecium strain specifically. | + | Aptamers are short single-stranded DNA or RNA oligonucleotides that can be selected to a wide variety of targets. They are similar to antibodies through their affinity and specificity and show a high potential as diagnostic and therapeutic tools, with many advantages when compared with antibodies. BBa_K3176020 is a DNA aptamer selected against ''E. faecium'' strain specifically from a random DNA library, using the Whole-Cell SELEX process. Because of its specificity and its affinity, it can be used to detect living ''E. faecium'' cells in any clinical samples when coupled to a fluorophore. |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | We tested this aptamer and its ability to detect ''E. faecium'' strains by measuring a change in electric potential across the nanotubes. | |
| − | + | ||
| − | + | ||
| − | < | + | <span class='h3bb'>Sequence and Features</span> |
| − | + | <partinfo>BBa_K3176020 SequenceAndFeatures</partinfo> | |
| − | <partinfo>BBa_K3176020 | + | |
| − | + | ||
Revision as of 00:05, 22 October 2019
Specific aptamer for E. faecium
Aptamers are short single-stranded DNA or RNA oligonucleotides that can be selected to a wide variety of targets. They are similar to antibodies through their affinity and specificity and show a high potential as diagnostic and therapeutic tools, with many advantages when compared with antibodies. BBa_K3176020 is a DNA aptamer selected against E. faecium strain specifically from a random DNA library, using the Whole-Cell SELEX process. Because of its specificity and its affinity, it can be used to detect living E. faecium cells in any clinical samples when coupled to a fluorophore.
Usage and Biology
We tested this aptamer and its ability to detect E. faecium strains by measuring a change in electric potential across the nanotubes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]