Difference between revisions of "Part:BBa K3317002:Design"

 
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===References===
 
===References===
 +
Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.

Revision as of 09:27, 15 October 2019


Protocatechuate decarboxylase (aroY) from Klebsiella pneumonia subsp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1045


Design Notes

sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided, a signal peptide () was added at its N terminal end with a flexible G4S linker


Source

ACCESSION BAH20873

References

Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.