Difference between revisions of "Part:BBa K3239000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | The PRM1 promoter is | + | The PRM1 promoter is homogeneous to the P. pastoris GS115 strain. It is self-upregulated by the Prm1 protein and downregulated by the Mit1 protein, both transcription factors homogeneous to the P. pastoris GS115 strain. |
===Reference=== | ===Reference=== |
Revision as of 10:21, 15 October 2019
Promoter for the Prm1 transcription factor in the P. pastoris GS115 strain
Promoter for the PRM1 gene in the P. pastoris GS115 strain. Upregulated by Prm1 and downregulated by Mit1.
Usage and Biology
The PRM1 promoter is homogeneous to the P. pastoris GS115 strain. It is self-upregulated by the Prm1 protein and downregulated by the Mit1 protein, both transcription factors homogeneous to the P. pastoris GS115 strain.
Reference
Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738
Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014
Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 293
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]