Difference between revisions of "Part:BBa K3239000"
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The promoter of the PRM1 gene in wildtype P. pastoris. Upregulated by Prm1 and downregulated by Mit1.
 
The promoter of the PRM1 gene in wildtype P. pastoris. Upregulated by Prm1 and downregulated by Mit1.
  
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===Usage and Biology===
 
===Usage and Biology===
 
The PRM1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulatory pathway of the expression of alcohol oxidase 1 (AOX1).
 
The PRM1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulatory pathway of the expression of alcohol oxidase 1 (AOX1).
 
Prm1 is one of the three critical transcription factors that upregulate the activity of the AOX1 promoter. When methanol is present, the activity of the PRM1 promoter is upregulated, and further amplified by the self-upregulation of Prm1 (Prm1 upregulates PRM1 promoter).
 
Prm1 is one of the three critical transcription factors that upregulate the activity of the AOX1 promoter. When methanol is present, the activity of the PRM1 promoter is upregulated, and further amplified by the self-upregulation of Prm1 (Prm1 upregulates PRM1 promoter).
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===Functional Parameters===
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===Functional Parameters===
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<partinfo>BBa_K3239000 parameters</partinfo>
 
<partinfo>BBa_K3239000 parameters</partinfo>
 
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Revision as of 07:18, 15 October 2019


Promoter for the Prm1 transcription factor in the P. pastoris GS115 strain

The promoter of the PRM1 gene in wildtype P. pastoris. Upregulated by Prm1 and downregulated by Mit1.

Usage and Biology

The PRM1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulatory pathway of the expression of alcohol oxidase 1 (AOX1). Prm1 is one of the three critical transcription factors that upregulate the activity of the AOX1 promoter. When methanol is present, the activity of the PRM1 promoter is upregulated, and further amplified by the self-upregulation of Prm1 (Prm1 upregulates PRM1 promoter).

Functional Parameters

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]