Difference between revisions of "Part:BBa K3140004:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
−
Psilocybe cubensis  
+
The native sequence for the putative cytochrome P450 monooxygenase PsiH (''Psilocybe cubensis'') was obtained from NCBI: GenBank accession [https://www.ncbi.nlm.nih.gov/nuccore/MF000993.1 MF000993.1]
  
 
===References===
 
===References===

Revision as of 06:41, 15 October 2019


PsiH (codon optimised) - Cytochrome P450 monooxygenase from Psilocybe cubensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was intended for use in pUS381 for initial analysis of protein expression and final expression. To achieve this, the part was ordered in a gBlock flanked by BamHI and XbaI recognition sequences to enable traditional restriction cloning into pUS381, along with the consensus Shine-Dalgarno sequence as an RBS upstream of the coding sequence.

Source

The native sequence for the putative cytochrome P450 monooxygenase PsiH (Psilocybe cubensis) was obtained from NCBI: GenBank accession MF000993.1

References