Difference between revisions of "Part:BBa K3239000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The PRM1 promoter is homogenous to the P. pastoris GS115 strain and is critical in the regulatory pathway of the expression of alcohol oxidase 1 (AOX1). | ||
| + | Prm1 is one of the three critical transcription factors that upregulate the activity of the AOX1 promoter. When methanol is present, the activity of the PRM1 promoter is upregulated, and further amplified by the self-upregulation of Prm1 (Prm1 upregulates PRM1 promoter). | ||
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Revision as of 07:14, 15 October 2019
Promoter for the Prm1 transcription factor in the P. pastoris GS115 strain
The promoter of the PRM1 gene in wildtype P. pastoris. Upregulated by Prm1 and downregulated by Mit1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 293
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]