Difference between revisions of "Part:BBa K3190109:Design"
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===References===
 
===References===
 +
Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943

Revision as of 09:17, 18 October 2019


Xenopus laevis lutropin-choriogonadotropic hormone receptor LHCGR CDS with Linker-superfolder GF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2179


Design Notes

The coding sequence for the receptor XLHCGR was codon optimised and fused with the nucleotides for the linker and superfolded GFP in the C-terminus (XLHCGR-Li-sfGFP) and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae. The construct was important to carry out colocalisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.

The linker was added in the primer design.

Linker sequence: GGAGGTGGCTCTGGTGGTGGATCA


Source

Xenopus laevis

References

Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. Science, 307(5715), 1625–1630. doi: 10.1126/science.1106943