Difference between revisions of "Part:BBa K143021"
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− | '''To get the highest level of translation from this RBS it must be connected to a coding region preceded by a coding region prefix<cite># | + | '''To get the highest level of translation from this RBS it must be connected to a coding region preceded by a coding region prefix<cite>#2</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency'''. |
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Revision as of 11:48, 30 September 2008
SpoVG Ribosome Binding Site (RBS) for B. subtilis
Description: SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
To get the highest level of translation from this RBS it must be connected to a coding region preceded by a coding region prefix#2. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
- 1 pmid=9669336
- 2 https://parts.igem.org/Assembly:RBS-CDS_issues
</biblio>