Difference between revisions of "Part:BBa K143032:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K143032 short</partinfo>
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===<big>EpsE Molecular Clutch Gene of ''B. subtilis''</big>===
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<br>
  
 
<partinfo>BBa_K143032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143032 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 
The epsE(aka yveO) sequence was located in the ''B. subtilis'' chromosome<cite>#1</cite> and the ''Pst''I restriction site removed before synthesis by GeneArt
 
The epsE(aka yveO) sequence was located in the ''B. subtilis'' chromosome<cite>#1</cite> and the ''Pst''I restriction site removed before synthesis by GeneArt
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===Source===
 
===Source===
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===References===
 
===References===
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<biblio>
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#1 pmid=9384377
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</biblio>

Latest revision as of 17:38, 18 September 2008

EpsE Molecular Clutch Gene of B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 762
    Illegal AgeI site found at 512
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The epsE(aka yveO) sequence was located in the B. subtilis chromosome#1 and the PstI restriction site removed before synthesis by GeneArt

Source

The epsE gene sequence was taken from the B. subtilis chromosome and was synthesised by GeneArt.

References

<biblio>

  1. 1 pmid=9384377

</biblio>