Difference between revisions of "Part:BBa K143012"

Revision as of 10:50, 19 September 2008

Promoter veg: Constitutive Promoter for B. subtilis

Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor, Sigma factor A 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1 pmid=6181373
2 pmid=6820069
3 pmid=14651647

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-Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B. subtilis''. Pveg contains binding sites for the ''B. sutbilis'' major sigma factor, Sigma factor A <cite>1</cite>. Pveg in ''B. subtilis'' utilises two binding sites to cause high expression of genes <cite>#2</cite>, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how <cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
+Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B. subtilis''. Pveg contains binding sites for the ''B. sutbilis'' major sigma factor, Sigma factor A <cite>1</cite>. Pveg in ''B. subtilis'' utilises two binding sites to cause high expression of genes <cite>#2</cite>, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how <cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
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