Difference between revisions of "Part:BBa K143031:Design"

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<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
  
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===Design Notes===
  
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===Design Notes===
 
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
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===Source===
 
===Source===
  
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase

Revision as of 15:43, 9 October 2008

Aad9 Spectinomycin Resistance Gene



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488

Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning

Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase