Difference between revisions of "Part:BBa K3110024"
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<partinfo>BBa_K3110024 short</partinfo> | <partinfo>BBa_K3110024 short</partinfo> | ||
| − | L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a strong promoter and a weak RBS. | + | This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a strong promoter and a weak RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying the strength of the promoter and RBS controlling their production. |
Revision as of 18:41, 18 October 2019
Strong Promoter Weak RBS lldP+lldD
This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a strong promoter and a weak RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying the strength of the promoter and RBS controlling their production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 327
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2863
Illegal AgeI site found at 463
Illegal AgeI site found at 926
Illegal AgeI site found at 1364
Illegal AgeI site found at 1523
Illegal AgeI site found at 1535 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2300