Difference between revisions of "Part:BBa K2989004"

 
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MiRNAs are a class of evolutionarily highly conserved single-stranded, non-coding small RNAs that are about 18 to 25 nt long. This kind of RNAs play important roles in many endogenic processes. Mostly, it functions as a post-transcriptional regulator of gene expression, which usually results in the silence of target gene. This process is commonly known as gene silencing.
  
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MiRNAs are a class of evolutionarily highly conserved single-stranded, non-coding small RNAs that are about 18 to 25 nt long. This kind of RNAs play important roles in many endogenic processes. Mostly, it functions as a post-transcriptional regulator of gene expression, which usually results in the silence of target gene. This process is commonly known as gene silencing. Our miR-HBsAg-125 can function at the 125bp of HBsAg and therefore, leads to the silence of HBsAg. In this case, we can ensure that HBsAg will not be expressed in target cells, since the gene will be silenced by our part.  
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===Usage===
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Our miR-HBsAg-125 can function at the 125bp of "Trojan horse" fragment (92 amino acid length HBsAg fragment).,and therefore, leads to the silence of our "Trojan horse" fragment. In this case, we can ensure that "Trojan horse" fragment will not be expressed in target cells, since the gene will be silenced by our part.
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===Usage and Biology===
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Revision as of 05:17, 18 October 2019


miR-HBsAg-125 MiRNAs are a class of evolutionarily highly conserved single-stranded, non-coding small RNAs that are about 18 to 25 nt long. This kind of RNAs play important roles in many endogenic processes. Mostly, it functions as a post-transcriptional regulator of gene expression, which usually results in the silence of target gene. This process is commonly known as gene silencing.

Usage

Our miR-HBsAg-125 can function at the 125bp of "Trojan horse" fragment (92 amino acid length HBsAg fragment).,and therefore, leads to the silence of our "Trojan horse" fragment. In this case, we can ensure that "Trojan horse" fragment will not be expressed in target cells, since the gene will be silenced by our part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]