Difference between revisions of "Part:BBa K143020"
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| − | < | + | ===<big>GsiB Ribosome Binding Site (RBS) for B.subtilis</big>=== |
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GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. | GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. | ||
Revision as of 13:44, 18 September 2008
GsiB Ribosome Binding Site (RBS) for B.subtilis
GsiB is an endogenous ribosome binding site from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis. GsiB is an endogenous ribosome binding site (RBS) from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
To get the higest level of translation from this RBS it must be connected to a coding region preceeded by a coding region prefix#1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
</biblio>