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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 11:38, 18 September 2008
Aad9 Spectinomycin Resistance Gene
Aad9 is the spectinomycin resistance gene from Enterococcus faecalis#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both B. subtilis and E. coli including pDP870#2, pCOMT-Kan#3 and pIEF16s#4
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 488
References
<biblio>
- 1 pmid=1659306
- 2 pmid=16997985
- 3 pmid=15060042
- 4 pmid=16714443
</biblio>