Difference between revisions of "Part:BBa K3187018"
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The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif and a N-terminal poly glycine motif <sup>[1][2][3]</sup>. The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction. | The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif and a N-terminal poly glycine motif <sup>[1][2][3]</sup>. The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction. | ||
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[1] Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering ChemBioChem | [1] Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering ChemBioChem | ||
Revision as of 14:20, 14 October 2019
PolyG Tag (GGGG) for Sortase-mediated Ligation
- Name: PolyG tag
- bp: 12
- Da: 300
- Properties: second sequence for Sortase A
Introduction:
The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif and a N-terminal poly glycine motif [1][2][3]. The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.
Sequence:
GGCggaggcggt
References:
[1] Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering ChemBioChem
[2] Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation
[3] Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]