Difference between revisions of "Part:BBa K3110000:Design"
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===Source===
 
===Source===
  
−
lldD is of Genomic origin and will be joined with Promoter RBS and Terminator using SOEing (Spliced Overlap Extension) PCR.
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The sequence was synthesized by Twist Biosciences.
  
 
===References===
 
===References===

Revision as of 12:22, 14 October 2019


lldD (L-Lactate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 586


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

The sequence was synthesized by Twist Biosciences.

References