Difference between revisions of "Part:BBa K3308081"
Line 5: Line 5:
 
N1 +MBP
 
N1 +MBP
  
−
[[File:T--Pittsburgh--nested inteins overview.png|900px|thumb|none|.|Figure 1. Nested Intein design overview]]
+
[[File:T--Pittsburgh--Nested intein overview.png|2000px|thumb|none|.|Figure 1. Nested Intein design overview]]
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 11:52, 14 October 2019


GB1-GTNPC-[NrdJ-1 N (1-4)]-[gp41-1 N]

N1 +MBP

File:T--Pittsburgh--Nested intein overview.png
Figure 1. Nested Intein design overview

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal BsaI.rc site found at 1227
    Illegal SapI.rc site found at 1527