Difference between revisions of "Part:BBa K2923021"
 
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<partinfo>BBa_K2923021 short</partinfo>
 
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Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923020</partinfo>
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Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923020</partinfo>
  
 
[[Image: tout Ms2-MS2-Aptazyme-PP7-PP7.jpg|500px|thumb|center| Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7
 
[[Image: tout Ms2-MS2-Aptazyme-PP7-PP7.jpg|500px|thumb|center| Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7

Latest revision as of 17:19, 20 October 2019


MS2-MS2-Guanine inactif aptazyme-PP7-PP7

Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923020

Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7

This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923020

Figure 2: Schematic graphic of inactive aptazyme. Inactive aptazymes (lost self-cleavage capability) active BH3 system independently of ligand presence


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]