Difference between revisions of "Part:BBa K2923008"

Revision as of 08:12, 14 October 2019

Guanine inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003

Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923003</partinfo>
-[[Image: Gua active et inactive.jpg|300px|thumb|center|Figure: Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
+[[Image: Gua active et inactive.jpg|300px|thumb|center|Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
 ]]